Tacrolimus and sirolimus decrease oxidative phosphorylation of isolated rat kidney mitochondria

Modelling of oxidative phosphorylation in isolated mitochondria and intact cells

Energetics and stoichiometry of oxidative phosphorylation from NADH to cytochrome c in isolated rat liver mitochondria.

The reaction 3-hydroxybutyrate (3-OH-butyrate) + 2 cytochrome c + ~ + 2 ADP + 2 Pi acetoacetate + 2 cytochrome e+’ + 2 ATP was used to study the energy relationships of electron transfer in the forward and reverse directions in suspensions of isolated rat liver mitochondria. Reduction of cytochrome c was monitored continuously at 550-540 nm while aliquots were taken to measure transmembrane pH ...

toxicity of depleted uranium on isolated rat kidney mitochondria

Decreased cytochrome c mediates an age-related decline of oxidative phosphorylation in rat kidney mitochondria.

Kidney function declines with advancing age and mitochondria have been implicated. In the present study we have examined the integrated function of mitochondria isolated from kidneys of 6- and 24-month-old Fischer 344 rats. OXPHOS (oxidative phosphorylation) of intact mitochondria and cytochrome c oxidase activity in permeabilized mitochondria were determined with polarographic assays. The acti...

Protective Effect of Captopril against Doxorubicin-Induced Oxidative Stress in Isolated Rat Liver Mitochondria

      Doxorubicin (DOX) is an anthracycline antibiotic that has been used for a long time in therapy of an array of human malignancies either alone or in combination with other cytotoxic agents. The dose-dependent cardiotoxicity of DOX significantly limits its anticancer efficacies. Oxidative stress caused by enhanced production of reactive oxygen species is an important contributor to DOX mito...